Journal: Oncology Letters

Article Title: Dpy-19 like 3-mediated C -mannosylation and expression levels of RPE-spondin in human tumor cell lines

doi: 10.3892/ol.2017.6465

Figure Lengend Snippet: RPESP is expressed in certain colon cancer cell lines. (A) Expression of RPESP mRNA in human tumor cell lines. Total RNAs were isolated from A549 lung adenocarcinoma, ES2 ovarian cancer, HepG2 hepatoma, HT1080 fibrosarcoma, HT29 colon cancer, Jurkat leukemia, LNCaP prostate cancer and WM266-4 melanoma cells and RT-sqPCR analysis was performed. (B) Expression of RPESP mRNA in human colon cancer cell lines. Total RNAs were isolated from human colon cancer cell lines, HT29, COLO 205, CW-2, HCT116 and LoVo, and RT-sqPCR analysis was performed. RT-sqPCR, reverse transcription-semi-quantitative polymerase chain reaction; RPESP, RPE-spondin.

Article Snippet: Human ES2 (which was kindly donated by the Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan) ovarian cancer and HCT116 (RIKEN BioResource Center) colon cancer cell lines were cultured in DMEM supplemented with 10% (v/v) heat-inactivated FBS, 100 U/ml penicillin G, 100 mg/l kanamycin, 600 mg/l L-glutamine, and 2.25 g/l NaHCO 3 at 37°C in a humidified incubator with 5% CO 2 .

Techniques: Expressing, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Stem Cell Research & Therapy

Article Title: Efficacy and safety of mesenchymal stem cell therapy for ovarian ageing in a mouse model

doi: 10.1186/s13287-024-03698-0

Figure Lengend Snippet: Tumorigenicity test of orthotopic transplantation of MSCs. A Flowchart overview of the establishment of an orthotopic xenograft tumour model in mice. B Flowchart overview of the evaluation of the tumorigenic potential of MSC transplantation. C Photographs and pathological section images of xenograft tumours established in situ. D Representative image of mice in the ES-2 injection group. E Photographs of ovaries in the AD-MSC injection group. F Photographs of ovaries in the UC-MSC injection group. G Representative pathological images of ovaries and other organs in the MSC transplantation group

Article Snippet: The ovarian cancer cell line ES-2 (iCell-h060, iCell) was used as a positive control for tumorigenicity detection.

Techniques: Transplantation Assay, In Situ, Injection

Journal: Drug Design, Development and Therapy

Article Title: Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers Cells

doi: 10.2147/DDDT.S258811

Figure Lengend Snippet: ( A and B ) The cell viability of human OC cells HEY and ES2 under the treatment of 1, 2 , and 3 at different concentration for 24 and 48 hours, respectively. ( C ) The cell viability of bronchial epithelial cell line 16HBE under the treatment of 1, 2 , and 3 at different concentration for 48 hours, respectively. Data are presented as mean ±SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, versus the control group.

Article Snippet: Human OC cell lines HEY and ES2, and human bronchial epithelioid cell lines 16HBE were purchased from Beyotime Institute of Biotechnology (Shanghai, China).

Techniques: Concentration Assay, Control